Effect of tea polyphenols as an antioxidant on pork for frying at different temperatures and times.

The aim of this study was to investigate the effect of frying on the antioxidant properties of tea phenols added to pork. The antioxidant capacity of tea polyphenols with different concentrations was tested using different assays including total antioxidant capacity (T-AOC) (FRAP method), thiobarbituric acid reactive substance, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) radical scavenging. Our results indicated that tea polyphenols have a great antioxidant capacity and that a high frying temperature causes fat oxidation. Our study confirmed that DPPH assay is more suited to lipophilic compounds or compounds with high lipid content. In a frying temperature of 180°C, the DPPH-free radical scavenging ability of pork was not decreased. Further experiments remain necessary to explore specific temperatures with the same results. This study provides new process parameters and new references for processing techniques of healthy and high-quality pork products.

Role of liver fatty acid binding protein in hepatocellular injury: effect of CrPic treatment.

This study was designed to investigate the molecular mechanisms of chromium picolinate (CrPic, Fig. 1) hepatoprotective activity from alloxan-induced hepatic injury. Diabetes is induced by alloxan-treatment concurrently with the hepatic injury in mice. In this study, we investigate the protective effect of CrPic treatment in hepatic injury and the signal role of liver fatty acid binding protein in early hepatocellular injury diagnostics. In this study, alanine aminotransferase (ALT; EC 2.6.1.2) and aspartate aminotransferase (AST; EC 2.6.1.1) levels in the alloxan group were higher 71% and 50%, respectively, than those of the control group (ALT: 14.51±0.74; AST: 22.60±0.69). The AST and ALT levels in CrPic group were of minimal difference compared to the control groups. Here, CrPic exhibited amelioration alloxan induced oxidative stress in mouse livers. A significant increase in liver fatty acid-binding protein (L-FABP) was observed, which indicates increased fatty acid utilization in liver tissue [1]. In this study, the mRNA levels of L-FABP increased in both the control (1.1 fold) and CrPic (0.78 fold) groups compared the alloxan group. These findings suggest that hepatic injury may be prevented by CrPic, and is a potential target for use in the treatment of early hepatic injury.

Hepatoprotective activity of CrPic against alloxan-induced hepatotoxicity in mice.

This study focused on oxidation hepatic injury induced by alloxan treatment in mice and the hepatoprotective effect of chromium picolinate (CrPic) against such injury. The mice were randomly divided into three groups (control, alloxan, and CrPic). The CrPic mice were given Cr(3+) (40 µg/kg bm/day), and other mice were given equivalent intragastric doses of water every day. After 4 weeks, the groups alloxan and CrPic were treated with alloxan (40 mg/kg/day) through intraperitoneal injection daily for 6 days. Biochemical and enzymatic characteristics were assayed in these animals. The MDA levels of the control and CrPic groups were 33.93 % and 28.45 % lower, respectively, than that of the alloxan group. The levels of superoxide dismutase (SOD) and GSH-Px in the alloxan group were 15.30 % and 15.69 % higher, respectively,than those of the control group. Both the SOD and GSH-Px levels of the control and CrPic groups were about the same. Levels of CuZnSOD mRNA of the control and CrPic groups were 0.27 fold and 1.03 fold lower than in the alloxan group. The transcription levels of GSH-Px in the control and CrPic groups were 1.57 fold and 0.99 fold below those of the alloxan group. These results show that significant hepatic injury can be induced by alloxan treatment in mice; in addition, CrPic may be useful in health products meant to treat human liver disease.